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Old 05-22-2015, 11:58 PM   #11
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,226
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Quote:
Originally Posted by pmiguel View Post
Does Agilent actually sell this Vibhar Tn kit?

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Phillip

They offer two transposase based kits one for SureSelect QXT capture and another for WGS library prep. There is no info in manuals on transposase used, but for sequencing one must use custom reads and index sequencing primers and use “CTGTCTCTTGATCACA” for adapter trimming which could be full or partial recognition sequence of transposase. Nucleotide BLAST for adapter sequence brings up lots of hits.
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