Thread: Demultiplexing
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Old 09-19-2015, 09:25 AM   #3
pingu
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Location: milan

Join Date: Jul 2015
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Thank you for your help, I know the barcodes and they are at the of the reads (last 10bp)
For example, this read is not assigned:
@JBMBIZC01CKLPI
CACTGTAGAAGACTCGGCAGCATCTCCATGAGCCAGTATTGAAAATGTTGAAGATCAAAAAA
CACTAAGTTTTTCCAAAGTTAATATCCAATGTAAAAGATAGCAAATGCATACCCACAAACTGTA
AATGAAGATATTTGCGTTGAGGAACTTGTGACTAGCTCTTCACCCTGCAAAAATAAAAATGCA
GCCATTAAATTGTCCATATCTAATAGTAATAATTTTGAGGTAGGGCCACCTGCATTTAGGATAG
CCAGTGGTAAAATCGTTTGTGTTTCACATGAAACATTAAAAAAGTGAAAGACATATTTACAGA
CAGTTTCAGTAAAGTAATTAAGGAAAACAACGAGAATAAATCAAAAATTTGCCAAACGAAAA
TTATGGCAGGTTGTTTGGAGAACAGTGACGATCGCCTACAGTGCT

The barcodes of the reads is: AGCACTGTAG and it is a complementary reverse: CTACAGTGCT,
I have MID at the start of the reads (5') e at the end (3'), the barcode splitter tool checks MID at 5' and I have allowed 2 mismatches.

Last edited by pingu; 09-19-2015 at 01:28 PM.
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