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Old 10-31-2018, 03:01 AM   #7
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,315

Just looking at this ancient thread that mcheckula revived. The answer I gave in 2013 misses the actual issue here. The "signal in the fast region" is the actual 25 nt standard peak. The software has incorrectly identified peaks inside the 5S region as being the 25 nt standard. So the best fix is to set the real 25 nt peak as the lower size marker.
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