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Old 10-31-2018, 03:08 AM   #9
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Location: Purdue University, West Lafayette, Indiana

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Originally Posted by mchekula View Post
Hi, I have a large peak in 5S region, larger than rRNA peaks, but RIN calculated based on 28S/18S ratio looks OK (8-8.5). RNA isolated TRIfast reagent. Any idea what it could be? Thanks!
Most likely it is a mixture of small RNAs -- 5S, tRNA, etc. "TRIfast" I have not heard of, but I presume it is another version of "Trizol", the commercial acid phenol reagents. For whatever reason, acid phenol methods seem to be extremely efficient at retaining low molecular weight RNAs that other methods mostly do not retain.

That said, I don't know that I've ever seen the 5S, etc. peak that large. So there might be something else going on.

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