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Old 12-03-2018, 02:49 AM   #7
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,315
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Quote:
Originally Posted by kgoglin View Post
We notice the same thing. Our qPCR concentrations are much less than Bioanalyzer concentrations.

I think the ligation efficiency of the adapters is low.

Have you had any progress on this issue since April?
Impossible to guess what "much less" means in this context. The previous poster mentioned 100x less -- that is clearly an issue.

Anyway, you will want to contact Illumina technical support. That is what they are there for.

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Phillip
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