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Old 05-23-2019, 07:15 AM   #106
Junior Member
Location: Europe

Join Date: Feb 2019
Posts: 4

Hi all,

I was wondering why the default for spantiles is set to false. If a read for instance has coordinates (1000,1000) and the dupedist is set to 2500, (see sketch attached), there's a possible overlap with 3 other tiles. So even if it's not a NextSeq, but a HiSeq4000 for instance, there are no tile-edge duplicates, however there's still a possibility that optical duplicates end up on neighboring tiles (or even further). Can anyone elucidate on this?

Thanks in advance!

Attachment: The dot represents the "original read", the circle represents the distance of 2500 around the "original read". Rectangles represent tiles.
Attached Images
File Type: png Screenshot from 2019-05-23 17-11-08.png (4.3 KB, 2 views)

Last edited by DCZ; 05-23-2019 at 07:27 AM.
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