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Old 08-05-2019, 05:10 PM   #1
SDPA_Pet
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Location: US

Join Date: Apr 2013
Posts: 222
Default Illumina output questions

Hello, I am gonna do some Illumina 2X300bp amplicon sequencing. I suppose the platform (MiSeq) will run 600 cycles and time fixed. I just recently notice on the official website

https://www.illumina.com/systems/seq...elect-language

It says the running time is 455 hours? It seems to me that the running time range is quite large? What's the difference if you run 4 hours or 55 hours. some questions:

1>Let's both 4 hours and 55 hours will give same length of reads.(In my case is 300bp). However, 55 hours will generate more reads (total number) than 4 hours.

2> Or 4 hours will give you shorter reads (e.g., 100bp) and lower number of reads.

3>Would 4 hours and 55hours running time used same protocols and same amount of regents?

4>Do you know how long normally a sequencing center will run? I would like them to run longer? Would be cost more? How should I tell them? Just simply say run as long as you could?

Thanks,
Ben
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