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Old 09-14-2019, 01:24 AM   #20
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,226
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Regardless of size distribution, library does not seem to have typical periodicity expected in ATAC-seq libraries indicating that there was ambient DNA in nuclei stock or nuclei disintegrated during transposition. Presence of 74bp fragment also raises question about accuracy of size selection.
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