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Old 10-13-2012, 12:00 PM   #1
proteomania
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Location: France

Join Date: Sep 2010
Posts: 11
Default 3'End polyA artefact in illumina TruSeq Small RNA Seq

Hi All,

I've been using Illumina TruSeq Kit to prepare Small RNA libraries. I found a small RNA population (non-miRNA) has about 1 to 8 extra As at their 3'end (mismatch to genome). I was wondering could this be the artefact of the TruSeq Kit or adaptor trimming?

Thanks.
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