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Old 07-27-2017, 02:35 AM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,189
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I do not see any adapter-dimer in library trace. qPCR melt analysis is not required for library quantification. Two melt peaks in input library could be due to high diversity of the library while the Chip libraries are less diverse as expected.

I wonder if you could you elaborate what “mess”” means in this context.
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