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Old 11-28-2011, 06:18 AM   #5
papori
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Location: berd

Join Date: Dec 2010
Posts: 179
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ok, thank you phillip, i think i got it.

so if i undersood tou right.
when converting mRNA to cDNA, i can get the full length of the mRNA as cDNA.
but after, when i want to create library(probably for PCR amplification) i have to break the cDNA because DNA polymerase has limit around 3K bases.

so, when i want to amplificate the library that i just broke to fragments, i must use random primers and not just polyT?

thanks
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