hello everyone,
I would like to ask one qus: why am I getting 3'adapter sequences in R1 and R2 reads (as according to seq protocol machine only generates seq of the fragment between the adapters and not the flanking adapters)??
Also, is it normal to encounter abundance of 3'adapters in the reads than 5'. if so, why?
I would like to ask one qus: why am I getting 3'adapter sequences in R1 and R2 reads (as according to seq protocol machine only generates seq of the fragment between the adapters and not the flanking adapters)??
Also, is it normal to encounter abundance of 3'adapters in the reads than 5'. if so, why?
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