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Old 07-14-2016, 06:44 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
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One has to take into account size distribution CV, reproducibility% and accuracy% for each Cassette type. Loading quantity, structure of DNA (blunt ended, Y shape, staggered ends) also can affect the size range distribution. On top of all of these also BA has its own specifications and deviation from them also will affect the sizing.

Your example of tight setting at 370bp will collect fragments in 337-403bp. If you use range setting of 350-390 it will not collect 370bp as that setting is narrower than tightest setting possible for which the software gives a narrow warning.

I agree with Sage explanation and their platform is much better than another one that I have tried.
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