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Old 07-12-2019, 12:54 AM   #1
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Location: US

Join Date: Apr 2013
Posts: 222
Default Did my sequencing center did a sloppy job?

Hello, I have just got my 16S rRNA amplicon sequencing back. We use typical Earth Microbiome Project primers 515F-806R, which is about 290bp size of amplicon.

We requested MiSeq 300bp X 2 pair-end sequencing. We got to files back R1.fastq and R2.fastq. I just checked these 2 files. The average of raw reads in these two files is 150 bp?

Did my sequencing center do a sloppy job? or they used wrong chemistry, kit?

I suppose my forward read and reverse read can be as long as to 290 bp, if they do 300bp X2?
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