The problem has been solved, thanks a lot!
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they are quite robust, I have been freezing and thawing them probably 10 times or more and they were still fine. Unless you are using 50 ng of input DNA for your Nextera library prep, you have a huge excess of index primers anyway, so if part of them went bad, you are still safe.
We use the Nextera XT kit, start from <500 pg for tagmentation and dilute the primers 1:5 to ame them last longer. And, even then, if we don´t do a bead clean up afterwards, we still see a huge peak of unused adaptors in the prep.
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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03-22-2024, 06:39 AM -
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