Tweet
When my Pacbio sequencing was done I ended up with 18 subreads that each contained xxxx number of sequences (6,431,149 sequences in total for all 18 subreads). Due to particular reasons of having an allocation at a super computer I was having troubles running the Canu command that encompasses all three stages: correct, trim, assemble. So what I decided to do was individually execute the Canu-correct command on each of the 18 subreads. So now I have a list of 18 subreads that have been corrected. My questions is would there be a difference if I would have executed the Canu-correct command on one single file that was a concatenation of all 18 subreads? I ask because I am going to do some downstream analysis with the corrected reads and want to make sure that I am doing this right. Thank you!
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment