Hi everyone,
We are getting some weird results when running RNA-seq libraries on Agilent Bioanalyzer.
The libraries show a peak at the expected length, but the peak itself is very noisy, with a sort of banding pattern:
We're using TruSeq RNA v.1 kit, following the protocol (except size-selection step before PCR enrichment, but we've seen the same thing without size selection). All libraries generated in the same batch do not display these strange peaks, some have a nice and smooth peak at the expected size.
Do you have any idea what could be going wrong?
Thank you very much in advance!
We are getting some weird results when running RNA-seq libraries on Agilent Bioanalyzer.
The libraries show a peak at the expected length, but the peak itself is very noisy, with a sort of banding pattern:
We're using TruSeq RNA v.1 kit, following the protocol (except size-selection step before PCR enrichment, but we've seen the same thing without size selection). All libraries generated in the same batch do not display these strange peaks, some have a nice and smooth peak at the expected size.
Do you have any idea what could be going wrong?
Thank you very much in advance!
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