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Old 02-19-2014, 11:38 AM   #1
Location: St. Louis, MO

Join Date: Apr 2013
Posts: 17
Default Differential gene expression across life cycle with DESeq2

I'm trying to study gene expression across the life cycle of a nematode using DESeq2. We've sequenced eggs, L1, L2, L3, L4, L5, and adults. I've figured out how to do stage v. stage comparisons (e.g., egg v. L1, L1 v. L2, L2 v. L3, etc). Now, I'd like to generate a list of genes whose expression fluctuates at some point in the life cycle -- between any of the stages. I'd appreciate some advice on how to tease this out.
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