Hello everyone!
I am pretty new on this forum, and also in bacterial genome assembly. So you can expect I will have a lot of questions in the next few weeks (or months).
My first question is about a bias in the GC content in the first 10 bases of my reads. These reads are from a bacterial genome sequenced with Illumina. I read a lot about a bias like this in illumina, about a random priming not so random, but of course, it's for RNA seq, and I do not understand why it happens with genomic data...
I join a picture. I guess it's not a big deal, but i would like to understand.
Is anyone can help me to figured out what happen in my reads?
Thanks a lot
Ben
I am pretty new on this forum, and also in bacterial genome assembly. So you can expect I will have a lot of questions in the next few weeks (or months).
My first question is about a bias in the GC content in the first 10 bases of my reads. These reads are from a bacterial genome sequenced with Illumina. I read a lot about a bias like this in illumina, about a random priming not so random, but of course, it's for RNA seq, and I do not understand why it happens with genomic data...
I join a picture. I guess it's not a big deal, but i would like to understand.
Is anyone can help me to figured out what happen in my reads?
Thanks a lot
Ben
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