Dear All,
We have carried out RNA-seq using Illumina. My question is If I have a transcript of size say 600 amino acids ( =1800bp), then when I get the possible ORFs for that transcript, how can I decide that which ORF is the best candidate to be a putative gene?
Also, to let you know further info, this transcript did not give any similarity to existing proteins from the nearby species. Henceforth, I would like to knw which ORF could be taken as putative candidate novel gene for my sepcies? Sounds tricky to me..
Thanks in advance!!
We have carried out RNA-seq using Illumina. My question is If I have a transcript of size say 600 amino acids ( =1800bp), then when I get the possible ORFs for that transcript, how can I decide that which ORF is the best candidate to be a putative gene?
Also, to let you know further info, this transcript did not give any similarity to existing proteins from the nearby species. Henceforth, I would like to knw which ORF could be taken as putative candidate novel gene for my sepcies? Sounds tricky to me..
Thanks in advance!!
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