Hi all,
I am hoping someone would give me real help, asking for true help. I am facing big problem making chip seq library by illumina tru-seq kit. My target gene is TF, I fused 3Xflag to my protein, used 50 million cells for CHIP. Before library preparation I did Q-pcr to validate whether my chip is working, my find was that my positive region was enriched by CHIP. But I always failed in making library, very low concentration of my library. Does anyone know how to improve in my case? Need help. Thanks so much.
I am hoping someone would give me real help, asking for true help. I am facing big problem making chip seq library by illumina tru-seq kit. My target gene is TF, I fused 3Xflag to my protein, used 50 million cells for CHIP. Before library preparation I did Q-pcr to validate whether my chip is working, my find was that my positive region was enriched by CHIP. But I always failed in making library, very low concentration of my library. Does anyone know how to improve in my case? Need help. Thanks so much.
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