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Old 01-15-2015, 07:53 PM   #24
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,182
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Quote:
Originally Posted by HeinKey View Post
Can these new flow cells still be overloaded, or has Illumina created the ideal clustering procedure? Just load your library at high concentration for maximal occupance of the flow cell "wells" and skip qPCR and titration.....
Anything known about the clustering procedure yet?
They still need to be quantified. Assuming similarity of HiSeq 4000 flow cell to HiSeq x, they can be overloaded which will reduce the reads %PF. Low loading will increase the number of duplicate reads. Illumina manual also states that underloading can reduce or result in unacceptable %PF. Loading is depend on library type (Nano or PCR-free).
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