Has anyone tried to run an expired MiSeq reagent kit? I've been working on developing an amplicon sequencing protocol, and the libraries look good through the KAPA quantification. Now I want to test them on the MiSeq without spending $1000 on a kit. We have an expired kit that we can't use for an actual, paying investigator so I wanted to at least try it out on my amplicons. Does anyone know if there's an expiration date built into the RFID tag? Our FAS didn't know.
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I've tried using expired kits before and the instrument does let you run them, but the read quality does seem to be a bit worse than a new kit. If you have nothing to lose other than a few hours of your time, I would just try it and see how things go.
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We have run a couple of kits roughly 6 months out of date. The cluster density was a little low but the data quality was really good.
It would be good to know what concentration you load and what cluster density you get as we have another expired kit waiting for trial samples to be run on it.
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Agreed
We've run quite a few 'expired' kits...
Never had a problem at all...Data Look great.
We cut anything with a Qscore <30, though...but that yield is still ~85% of total reads.
SInce the kits are shipped on dry ice, we store our kits at -80 for bulk storage, and I bring about 4 at a time into a -20...easier to access, and a bit faster to thaw out. I figure that the reagents are fine at -80 (in reality, -70).
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sorry to dredge up an old thread but i wanted to tack on another data point.
this week i ran a 1-2 month expired (chip was 1 month, reagents were 2 months somehow) MiSeq v3 150-cycle kit and it worked beautifully despite the dire warning screen i had to click through.
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Thank you for your reply. My main concern is whether the reagent kit serves only a single run or else, how much sequencing runs (different/independent) will I be able to do with the purchase of a Miseq reagent kit v2. Does the reagent cartridge serve a single sequencing run only? I'm sorry if I sound a little detached from reality. It's just that I'm learning about Illumina solutions as of now (for a theoretical project) and I lack lab experience regarding NGS.
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