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Old 06-28-2013, 10:33 AM   #3
Junior Member
Location: USA

Join Date: Jun 2013
Posts: 2

Thanks flxlex for your response. A week ago, I already read the link you sent.

I decide to use only 500bp since i am going to do community analysis. I think the higher number of 500 short sequence is better than the small sequence of full length.
I do trust the barcodes suggested by PacBio, I only concern about the barcoded primers (primers with bacode attached). How can i decide based on the test for self dimer, hairpin...?

Or Can i pick any recommended barcode for my primers?
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