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Old 09-21-2013, 06:09 PM   #4
yaximik
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Location: Oregon

Join Date: Apr 2011
Posts: 205
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All right, I did another run with the same library using the first protocol, but loaded the flow cell at ~33 pM instead of 25 pM as in the previous run. I got density up to 1144 with 85.2% PF as compared to density of 928 with PF 88% at 25 pM library load. Looks like the protocol 1 is the way to go.

Last edited by yaximik; 09-21-2013 at 06:13 PM.
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