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Old 09-22-2013, 11:02 PM   #5
MadsAlbertsen
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Location: Denmark

Join Date: Aug 2010
Posts: 26
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Quote:
Originally Posted by yaximik View Post
All right, I did another run with the same library using the first protocol, but loaded the flow cell at ~33 pM instead of 25 pM as in the previous run. I got density up to 1144 with 85.2% PF as compared to density of 928 with PF 88% at 25 pM library load. Looks like the protocol 1 is the way to go.
Nice to see that you got it to work. We use the new loading reccomendations of 20 pM. However it is very difficult to estimate the right concentration of the samples due to the differences in insert length although we run all samples on a TapeStation. We are usually happy if we hit +/- 20 % of the density we aimed for.

rgds
Mads
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