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Old 09-29-2013, 09:47 AM   #2
bnmtthws
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Location: New York City

Join Date: Mar 2011
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when we've had similar problems with a barcoded primer set i've simply performed two rounds of PCR - use the primers without barcodes first, and the reamplify with the barcoded primers.

if you want to keep cycle numbers down, you should be able to get away with ~ 10-20 cycles for the first PCR and ~10-20 for the second depending on efficiency.
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