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Old 09-30-2013, 06:05 AM   #4
Junior Member
Location: New York City

Join Date: Mar 2011
Posts: 4

i hear you - good luck!

at this point, i don't even bother running out the 1st PCR on a gel - we'll run the results of the 2nd pcr through the qubit and bioanalyzer to check sizing and concentration, and have had pretty good luck so far.

another option is to remove half of the reaction after, say, 10 or 15 cycles and let the rest go to completion to run on a gel - this will give you confirmation that the reaction is 'working' but allow you to use the portion of the reaction that you removed as the template for the next round.
bnmtthws is offline   Reply With Quote