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Old 12-10-2013, 04:51 AM   #1
Location: Vancouver

Join Date: Dec 2013
Posts: 23
Default Bioanalyzer: What are these huge fragments (after Illumina TruSeq RNA sample prep)?

Hi everybody,

This has been my second time doing a library prep using total RNA from plants. I used the Illumina TruSeq RNA sample prep kit v2.
Last time, everything worked all fine but this time after the final pcr step, the bioanalyzer results all show a huge peak at the beginning of the curve (see attachment).

Can anybody tell me what this is, where it could have come from and what I could do next?

Attached Images
File Type: png Bioanalyzer.png (49.1 KB, 160 views)
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