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Old 01-15-2014, 08:46 AM   #2
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Location: East Coast USA

Join Date: Feb 2008
Posts: 7,076

First part could be explained by having adapter/primer dimers without any insert.

As for trimming give "trimmomatic" ( or cutadapt ( (http://www.bioinformatics.babraham.a...s/trim_galore/) a try. Recent comparison of trimmers available
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