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Old 09-09-2010, 12:06 PM   #3
Location: Pittsburgh, PA

Join Date: May 2010
Posts: 22

What it has to do with my analysis? The result is straight from the software SOLiD SAGE Analysis tool. In the file it produces read ids for the tags are mentioned. I counted the unique set from those read ids and divided it by the unique set of read ids from the read file to get the percentage of reads mapped to human mRNA obtained from Refseq database.
I will still BLAT some of them and see where they are mapping
If they are not mapping to mRNA then something should be with the reads generated by SOLiD SAGE run
rahilsethi is offline   Reply With Quote