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Old 02-04-2011, 07:58 AM   #5
HESmith
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Location: Bethesda MD

Join Date: Oct 2009
Posts: 509
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1. You can use custom primers for sequencing. The Tm should be similar to the standard primer.

2. You can use different primers in different lanes of the flow cell.

3. Biased nucleotide composition in the first few cycles (I believe this number varies with different software versions) adversely affects cluster calling.

-Harold
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