Thread: extend sequence
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Old 08-24-2012, 12:16 PM   #1
mirex
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Location: sao paulo

Join Date: May 2011
Posts: 2
Default extend sequence

Hello,
I have paired end reads Illumina and I need to complete a sequence of bacteria, i have only 4kbp, I would complete the ends of a bacterial sequence, I have only 4kbp, for that I'm using Novoalign trying to lengthen the ends doing a loop with the previous results, anybody can give me better ideas?
I would also like to know what you mean trim consensus sequences to 4x coverage from pileup file. Sorry, I'm new in this work, how I do this?, delete lines that have a greater coverage to 4?, is there a program for this?
Thanks,
Mirex
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