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Old 12-27-2016, 03:52 AM   #1
krutipedali
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Location: Norway, Bergen

Join Date: Nov 2016
Posts: 3
Default On-Plate ATAC-seq

Hello,

Could anyone please drop any comments on libraries that i have prepared using on-plate ATAC protocol (DOI: 10.1186/s13059-015-0840-9). Libraries seem to be overtagmented, but i would like to receive any comment considering their sequencibility and further actions of optimising tagmentation protocol. This time i used standard NEXTERA protocol(2,5ul of enzyme) and 50000 HeLa cells.

Thanks in advance.
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File Type: pdf Bioanalyzer_ATAC_and_ChIP.pdf (1.81 MB, 88 views)
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