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Old 10-13-2016, 04:41 AM   #1
Sylv
Junior Member
 
Location: Norway

Join Date: Oct 2016
Posts: 6
Default Problems using Agencourt AmPure XP beads.

Hi.
I am testing some DNA samples for doing ddRAD sequencing, and I have a problem.
After restriction digest, I purify the reaction using Agencourt Ampure XP beads. When eluting the DNA in water, the beads are stuck to the wells, and very difficult to resuspend.

Have anyone experienced the same problem, and have som ideas as to what causes this? I was thinking the beads have been drying to long, but drying them for shorter time did not make any difference. A pH problem should affect binding of DNA to the beads, and not stick the beads to the wells right??

I hope someone have some advice for me
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