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Old 10-13-2016, 08:34 AM   #4
Location: United States

Join Date: May 2014
Posts: 40

What volumes are you working with? Diluting your sample may help with clumping produced by BSA or other components of your digest.

How long are you drying your beads for? Have you tried doing additional ethanol washes? If you're vortexing, are you then spinning down your elution volume before placing it on the magnet to prevent droplets from coating the sides of your wells?

As jdk787 said, though, this is only a problem if you have reduced yield or bead carryover.
adam.geber is offline   Reply With Quote