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Old 12-11-2016, 10:27 AM   #5
David Eccles (gringer)
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 823

You can create rarefaction curves to see if what you have is likely sufficient to describe the metagenomic profile.

The basic process is to remove reads and see if your calculation of the species diversity is similar. A low complexity sample will plateau at a low coverage, while the diversity of a high complexity sample will just keep increasing substantially with more reads.
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