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Old 05-07-2015, 06:04 PM   #1
Location: Davis, CA

Join Date: Mar 2015
Posts: 31
Default HiSeq3000/4000 duplicate removal

Hi All,

Our Illumina FAS mentioned that in underloaded lanes on the patterned flow-cells even PCR-free libraries can generate significant numbers of duplicates (clusters swapping over the "walls" into the next empty nanowell if given enough time?).
Would you know of any software that can analyze/remove duplicates based on the coordinate information in the read headers (without any alignments)?

Thanks in advance,

Last edited by DNATECH; 05-07-2015 at 07:01 PM.
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