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Old 01-25-2016, 04:52 PM   #27
Junior Member
Location: Arizona

Join Date: Nov 2015
Posts: 2

Just to add another voice, we noticed this on our validation runs at install in late Nov/December. Q30s dropping after 100 cycles of read 2, ending the run at ~40%. Error rates up above 7-8% as well. It was actually most obvious in the control lanes of PhiX that we ran. Seems like it must be a reagent issue, probably with enzyme or cleavage mix.
This thread just prompted me to email our FAS about it again. We didn't have a very satisfactory response from tech support, they said they hadn't seen enough 4K runs to determine what was "normal", but that our runs meet spec.
My PI said he's heard through the grapevine that this problem is widespread though - at Yale, Cornell, NYGC etc.

Last edited by neurula; 01-26-2016 at 09:22 AM.
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