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Old 05-21-2019, 08:43 PM   #2
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Location: US

Join Date: Dec 2010
Posts: 412

The presence of a prominent short fragment band indicates that degradation is not the problem. Bacterial RNA-seq obviously will not make use of poly-A enrichment and thus is not very sensitive to degradation anyhow.
We have not run into a 95 nt RNA band before though.
I would suggest to post the tapestation trace here.
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