Hello people,
I tried the ScriptSeq v2 RNA Library Prep Kit on the total RNA extract of a cerebrospinal fluid sample. The RNA amount was not measureable with our Qubit but I tried anyway. I followed the protocol, did the AMPure bead purification, ran 16 PCR cyles and got this massive 135b-peak in the bioanalyzer that towers everything.
Any idea what this could be? I'm super new to RNA seq and not yet familiar with all the intricacies and pitfalls of the steps, so any help is appreciated
Cheers!
I tried the ScriptSeq v2 RNA Library Prep Kit on the total RNA extract of a cerebrospinal fluid sample. The RNA amount was not measureable with our Qubit but I tried anyway. I followed the protocol, did the AMPure bead purification, ran 16 PCR cyles and got this massive 135b-peak in the bioanalyzer that towers everything.
Any idea what this could be? I'm super new to RNA seq and not yet familiar with all the intricacies and pitfalls of the steps, so any help is appreciated
Cheers!
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