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Old 05-27-2013, 06:16 AM   #4
jimmyinfrance
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Location: France

Join Date: May 2011
Posts: 16
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Here's a back-of-the-envelope calculation (i.e. it's rough).

Assuming that average amplicon size = 200 bp
you have 112 amplicons,
you want 100X seq coverage

= 2,2 mbp/individual (ie 200bp X 112 amplicons X 100 fold).

2,2 X 48 = 107 mbp total. ABout capacity of a 316 mbp

Very rough though so maybe overestimate needed coverage in order to achieve the minimum you need (ie difference in amplicons, barcodes etc).
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