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Old 06-23-2017, 01:04 AM   #1
KateShepard
Junior Member
 
Location: UK

Join Date: Apr 2016
Posts: 5
Default 16S sequencing tissue - host DNA contamination

Dear all,

We are planning to use human/mouse intestinal tissues/biopsies for V4 microbiome sequencing. I am worried that the large amount of host DNA from the tissue will result in amplification of host mitochondrial DNA.

Does anyone have experience of V4 sequencing of tissue samples or biopsies, and if so is there usually a high proportion of mitochondrial sequences present? Are there any strategies to reduce host mitochondrial amplification?

Thanks for your advice,
Kate
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