View Single Post
Old 07-08-2018, 12:11 PM   #123
Location: Thessaloniki, Greece

Join Date: Jul 2018
Posts: 12


"These steps replicate the QA protocol implemented at JGI for Illumina reads. There is a program “RQCFilter” which implements them as a pipeline, but that is not publically available because it has numerous hard-coded paths to reference datasets of contaminants."

It is in the bbtools files.

Nevermind! 1) Is it a good plan to normalise and error correct first BEFORE merging? 2) Do I need to follow a different approach at trimming and filtering short vs long mate pair reads (Nextera)?

Last edited by kokyriakidis; 07-08-2018 at 12:15 PM.
kokyriakidis is offline   Reply With Quote