Thread: FastQC problems
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Old 01-24-2020, 09:04 AM   #1
Robo
Junior Member
 
Location: Germany.Frankfurt

Join Date: Jan 2020
Posts: 3
Exclamation FastQC problems

Greetings to every one. Here we have another newbie who made the reckless decision to throw himself in bioinformatics . I was given certain sequences from nanopore and pacbio, so really long sequences. Managed the program to work correctly in the server as a perl script but when I run it with the data as an argument I get the following error

Exception in thread "Thread-1" java. lang. Out Of Memory Error: Java heap space
at uk.ac.babraham.FastQC.Utilities.QualityCount.<init>(QualityCount.java:33)
at
uk.ac.babraham.FastQC.Modules.Per Base Quality Scores. process Sequence (Per Base Quality Scores. jaVA:141)
at uk.ac.graham. FastQC.Analysis.Analysis Runner.run(Analysis Runner.java:89)
at java. lang.Thread.run(Thread.java:748)


The strange thing is that thru the same data it worked ones so either I have somehow damaged the data or I am doing something wrong without realizing it.

I would really appreciate some help and I thank you for stopping by and reading my post

Last edited by Robo; 01-25-2020 at 05:08 AM.
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