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Old 03-01-2012, 05:50 AM   #3
protist
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Location: Ireland

Join Date: Jan 2009
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In your 2 million reads you have to take into account whether the original RNA has been rRNA depleted or not. If your libraries are from total RNA and there was no ribosomal RNA depletion you will not get sufficient mRNA coverage in 2 million reads.

I agree PE is not required for all our bacterial libraries we find 42 cycles to be sufficient.

With regard to the biorep question, you are correct sampling from the same flask constitutes a technical replicate not a biological one.

Best of luck.
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