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Old 10-11-2012, 01:03 AM   #1
Location: Sheffield, UK

Join Date: Feb 2012
Posts: 18
Default Total RNA stability following extraction/isolation

Hi all,

I will be extracting total RNA from animal tissue using the RNeasy minikit. The final step of which is to elute in to RNase-free water.

The next step, following extraction/isolation, will be to gauge the quality/quantity before sending away for library preparation and RNA-seq.

I have seen that some researchers use either RNA stabilizing solutions (e.g. RNA secure) instead of water or add RNase inhibiting supplements to the RNase-free water.

What do people use on this forum? What are the pros and cons of replacing/adding to RNAse-free water? Are there any downstream considerations to take into account, for example do some of these chemicals inhibit some of the reactions taking place in library preparation or sequencing?

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