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Old 10-11-2012, 01:03 AM   #1
pig_raffles
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Location: Sheffield, UK

Join Date: Feb 2012
Posts: 18
Default Total RNA stability following extraction/isolation

Hi all,

I will be extracting total RNA from animal tissue using the RNeasy minikit. The final step of which is to elute in to RNase-free water.

The next step, following extraction/isolation, will be to gauge the quality/quantity before sending away for library preparation and RNA-seq.

I have seen that some researchers use either RNA stabilizing solutions (e.g. RNA secure) instead of water or add RNase inhibiting supplements to the RNase-free water.

What do people use on this forum? What are the pros and cons of replacing/adding to RNAse-free water? Are there any downstream considerations to take into account, for example do some of these chemicals inhibit some of the reactions taking place in library preparation or sequencing?

Thanks
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