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Old 10-16-2012, 04:52 AM   #6
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Location: Oregon

Join Date: Apr 2011
Posts: 205

RNA isolated with RNeasy kit is pretty stable at -20, that is if no RNAse is brought along the way, which is obviously the key issue. A slightly acidic pH, like 5-6 with a few mM sodium citrate helps too. If RNA is concentrated, the best way for me was just storing it in pure formamide diluting to working concentration in water. A few % fomamide in solution does not seem to hurt enzymatic activities. Also, RNA can be eluted directly from glass fiber with fomamide instead of water.
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