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Old 10-16-2012, 05:28 AM   #8
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317
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I think you want to fixate on Ethan's 2nd sentence:

Quote:
But it might depend on where you RNA is coming from.
Sure, pure RNA under mildly acid pH is stable at room temp. But those 2' hydroxyls are just aching to react with their adjacent phosphodiester bonds causing the formation of a cyclic '2-3' phosphate bond and cleaving the RNA polymer at that site. Increase the pH, the temp, add divalent cation, or, of course, an RNAse and it is goodbye intact RNA.

RNAse is a big problem both because there are likely multiple species of it present in every cell and your lab is likely replete with RNAseA (from the last plasmid or genomic DNA prep you or your colleague did) in the dust raining down on your bench.

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Phillip
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