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Old 09-06-2017, 05:19 AM   #4
Location: Canada

Join Date: Jun 2013
Posts: 48

You might want to compare the clustering at the top (where sample was loaded) versus the bottom.

With typical cluster generation there are more clusters at the top than the bottom of the lanes since DNA is bound as it flows over the cell and there is a decreasing amount of DNA library available for binding.

With over-clustering there are fewer clusters interpreted by the machine at the top since there are so many clusters close together and they are erroneously interpreted as fewer clusters at the top compared to the bottom of the cell.
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